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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
15/08/2022 |
Actualizado : |
01/12/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
PASSOS, J.R.S.; GUERREIRO, D.D.; KAMILA S. OTÁVIO; DOS SANTOS-NETO, P.C.; SOUZA-NEVES, M.; CUADRO, F.; NUÑEZ?OLIVERA, R.; CRISPO, M.; VASCONCELOS, F.R.; BEZERRA, M.J.B.; SILVA, R.F.; LIMA, L.F.; FIGUEIREDO, J.R.; BUSTAMANTE-FILHO, I.C.; MENCHACA, A.; MOURA, A.A. |
Afiliación : |
JOSÉ RENATO S. PASSOS, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; DENISE D. GUERREIRO, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; OTÁVIO, K.S., Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; PEDRO C. DOS SANTOS-NETO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; MARCELA SOUZA-NEVES, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; FEDERICO CUADRO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; RICHARD NUÑEZ?OLIVERA, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; MARTINA CRIPO, Unidad de Biotecnología en Animales de Laboratorio, Institut Pasteur de Montevideo, Montevideo, Uruguay.; FÁBIO R. VASCONCELOS, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; MARIA JULIA B. BEZERRA, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; RENATO F. SILVA, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), Ceará State University, Fortaleza, Brazil.; LARITZA F. LIMA, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), Ceará State University, Fortaleza, Brazil.; JOSÉ RICARDO FIGUEIREDO, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), Ceará State University, Fortaleza, Brazil.; IVAN C. BUSTAMANTE-FILHO, Laboratório de Biotecnologia, Universidade do Vale do Taquari, Lajeado, Brazil.; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; ARLINDO A. MOURA, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil. |
Título : |
How in vitro maturation changes the proteome of ovine cumulus-oocyte complexes?. |
Complemento del título : |
Volume 89, Issue 10, Pages 459 - 470October 2022 |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
Molecular reproduction and development, October 2022, Volume 89, Issue 10, pages 459-470. doi: https://doi.org/10.1002/mrd.23638 |
DOI : |
10.1002/mrd.23638 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 16 February 2022 | Accepted: 21 July 2022. -- Corresponding author: Moura, A.A.; Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil; email:arlindo.moura@gmail.com -- Funding: The experiments presently described were conducted at the facilities of the Instituto de Reproducción Animal Uruguay (Fundacion IRAUy, Montevideo, Uruguay) and at the Unidad de Biotecnología en Animales de Laboratorio (UBAL) of the Institut Pasteur de Montevideo, Uruguay. Specially, the authors thank Dr. Rosario Durán and Dr. Alejandro Leyva for kindly assisting us in the proteomic experiment. Financial support was provided by Fundacion IRAUy; PRONEX 02/2015 (Programa de Apoio a Núcleos de Excelência Pronex/Funcap/CNPq); The Brazilian Research Council-CNPq (grants # 313160/2017-1 and 438773/2018-7); Brazilian Commission for Higher Education (CAPES); Ceará State Foundation for the Support of Technology and Scientific Development (FUNCAP), Brazil. |
Contenido : |
Abstract: The present study evaluated the effects of in vitro maturation (IVM) on the proteome of cumulus-oocyte complexes (COCs) from ewes. Extracted COC proteins were analyzed by LC-MS/MS. Differences in protein abundances (p < 0.05) and functional enrichments in immature versus in vitro-matured COCs were evaluated using bioinformatics tools. There were 2550 proteins identified in the COCs, with 89 and 87 proteins exclusive to immature and mature COCs, respectively. IVM caused downregulation of 84 and upregulation of 34 proteins. Major upregulated proteins in mature COCs were dopey_N domain-containing protein, structural maintenance of chromosomes protein, ubiquitin-like modifier-activating enzyme 2. Main downregulated proteins in mature COCs were immunoglobulin heavy constant mu, inter-alpha-trypsin inhibitor heavy chain 2, alpha-2-macroglobulin. Proteins exclusive to mature COCs and upregulated after IVM related to immune response, complement cascade, vesicle-mediated transport, cell cycle, and extracellular matrix organization. Proteins of immature COCs and downregulated after IVM were linked to metabolic processes, immune response, and complement cascade. KEGG pathways and miRNA-regulated genes attributed to downregulated and mature COC proteins related to complement and coagulation cascades, metabolism, humoral response, and B cell-mediated immunity. Thus, IVM influenced the ovine COC proteome. This knowledge supports the future development of efficient IVM protocols for Ovis aries. © 2022 Wiley Periodicals LLC. MenosAbstract: The present study evaluated the effects of in vitro maturation (IVM) on the proteome of cumulus-oocyte complexes (COCs) from ewes. Extracted COC proteins were analyzed by LC-MS/MS. Differences in protein abundances (p < 0.05) and functional enrichments in immature versus in vitro-matured COCs were evaluated using bioinformatics tools. There were 2550 proteins identified in the COCs, with 89 and 87 proteins exclusive to immature and mature COCs, respectively. IVM caused downregulation of 84 and upregulation of 34 proteins. Major upregulated proteins in mature COCs were dopey_N domain-containing protein, structural maintenance of chromosomes protein, ubiquitin-like modifier-activating enzyme 2. Main downregulated proteins in mature COCs were immunoglobulin heavy constant mu, inter-alpha-trypsin inhibitor heavy chain 2, alpha-2-macroglobulin. Proteins exclusive to mature COCs and upregulated after IVM related to immune response, complement cascade, vesicle-mediated transport, cell cycle, and extracellular matrix organization. Proteins of immature COCs and downregulated after IVM were linked to metabolic processes, immune response, and complement cascade. KEGG pathways and miRNA-regulated genes attributed to downregulated and mature COC proteins related to complement and coagulation cascades, metabolism, humoral response, and B cell-mediated immunity. Thus, IVM influenced the ovine COC proteome. This knowledge supports the future development of efficient IVM protocols ... Presentar Todo |
Palabras claves : |
FOLLICLE; OVARY; OVINE; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; PLATAFORMA DE SALUD ANIMAL; PROTEINS; REPRODUCTION. |
Asunto categoría : |
-- |
Marc : |
LEADER 03782naa a2200409 a 4500 001 1063525 005 2022-12-01 008 2022 bl uuuu u00u1 u #d 024 7 $a10.1002/mrd.23638$2DOI 100 1 $aPASSOS, J.R.S. 245 $aHow in vitro maturation changes the proteome of ovine cumulus-oocyte complexes?.$h[electronic resource] 260 $c2022 500 $aArticle history: Received: 16 February 2022 | Accepted: 21 July 2022. -- Corresponding author: Moura, A.A.; Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil; email:arlindo.moura@gmail.com -- Funding: The experiments presently described were conducted at the facilities of the Instituto de Reproducción Animal Uruguay (Fundacion IRAUy, Montevideo, Uruguay) and at the Unidad de Biotecnología en Animales de Laboratorio (UBAL) of the Institut Pasteur de Montevideo, Uruguay. Specially, the authors thank Dr. Rosario Durán and Dr. Alejandro Leyva for kindly assisting us in the proteomic experiment. Financial support was provided by Fundacion IRAUy; PRONEX 02/2015 (Programa de Apoio a Núcleos de Excelência Pronex/Funcap/CNPq); The Brazilian Research Council-CNPq (grants # 313160/2017-1 and 438773/2018-7); Brazilian Commission for Higher Education (CAPES); Ceará State Foundation for the Support of Technology and Scientific Development (FUNCAP), Brazil. 520 $aAbstract: The present study evaluated the effects of in vitro maturation (IVM) on the proteome of cumulus-oocyte complexes (COCs) from ewes. Extracted COC proteins were analyzed by LC-MS/MS. Differences in protein abundances (p < 0.05) and functional enrichments in immature versus in vitro-matured COCs were evaluated using bioinformatics tools. There were 2550 proteins identified in the COCs, with 89 and 87 proteins exclusive to immature and mature COCs, respectively. IVM caused downregulation of 84 and upregulation of 34 proteins. Major upregulated proteins in mature COCs were dopey_N domain-containing protein, structural maintenance of chromosomes protein, ubiquitin-like modifier-activating enzyme 2. Main downregulated proteins in mature COCs were immunoglobulin heavy constant mu, inter-alpha-trypsin inhibitor heavy chain 2, alpha-2-macroglobulin. Proteins exclusive to mature COCs and upregulated after IVM related to immune response, complement cascade, vesicle-mediated transport, cell cycle, and extracellular matrix organization. Proteins of immature COCs and downregulated after IVM were linked to metabolic processes, immune response, and complement cascade. KEGG pathways and miRNA-regulated genes attributed to downregulated and mature COC proteins related to complement and coagulation cascades, metabolism, humoral response, and B cell-mediated immunity. Thus, IVM influenced the ovine COC proteome. This knowledge supports the future development of efficient IVM protocols for Ovis aries. © 2022 Wiley Periodicals LLC. 653 $aFOLLICLE 653 $aOVARY 653 $aOVINE 653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL 653 $aPLATAFORMA DE SALUD ANIMAL 653 $aPROTEINS 653 $aREPRODUCTION 700 1 $aGUERREIRO, D.D. 700 1 $aKAMILA S. OTÁVIO 700 1 $aDOS SANTOS-NETO, P.C. 700 1 $aSOUZA-NEVES, M. 700 1 $aCUADRO, F. 700 1 $aNUÑEZ?OLIVERA, R. 700 1 $aCRISPO, M. 700 1 $aVASCONCELOS, F.R. 700 1 $aBEZERRA, M.J.B. 700 1 $aSILVA, R.F. 700 1 $aLIMA, L.F. 700 1 $aFIGUEIREDO, J.R. 700 1 $aBUSTAMANTE-FILHO, I.C. 700 1 $aMENCHACA, A. 700 1 $aMOURA, A.A. 773 $tMolecular reproduction and development, October 2022, Volume 89, Issue 10, pages 459-470. doi: https://doi.org/10.1002/mrd.23638
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
25/09/2016 |
Actualizado : |
09/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
ARRUABARRENA, A.; BENITEZ-GALEANO, M.J.; GIAMBIASI, M.; BERTALMIO, A.; COLINA, R.; HERNÁNDEZ-RODRÍGUEZ, L. |
Afiliación : |
ANA ARRUABARRENA PASCOVICH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA JOSÉ BENÍTEZ-GALEANO, Laboratorio de Virología Molecular, Centro Universitario Regional Noroeste (CENUR Noroeste), Universidad de la República; MARIO ALEJANDRO GIAMBIASI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA MARIA BERTALMIO CASARIEGO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODNEY COLINA, Laboratorio de Virología Molecular, Centro Universitario Regional Noroeste (CENUR Noroeste), Universidad de la República; LESTER HERNÁNDEZ-RODRÍGUEZ, Instituto de Investigaciones en Fruticultura Tropical, La Habana, Cuba. |
Título : |
Application of a simple and affordable protocol for isolating plant total nucleic acids for RNA and DNA virus detection. |
Fecha de publicación : |
2016 |
Fuente / Imprenta : |
Journal of Virological Methods, 2016, v.237, p. 14-17. |
DOI : |
10.1016/j.jviromet.2016.08.011 |
Idioma : |
Inglés |
Notas : |
Article history: Received 30 May 2016 / Received in revised form 26 July 2016 / Accepted 14 August 2016 / Available online 16 August 2016. |
Contenido : |
ABSTRACT.
Standard molecular methods for plant virus diagnosis require the purification of RNA or DNA extracts from a large number of samples, with sufficient concentration and quality for their use in PCR, RT-PCR, or qPCR analysis. Most methods are laborious and use either hazardous and/or costly chemicals.Apreviously published protocol for RNA isolation from several plant species yields high amounts of good quality RNADNA mixture in a simple, safe and inexpensive manner. In the present work, this method was tested to obtain RNA-DNA extracts from leaves of tomato, potato and three species of citrus, and was compared with two commercial kits. The results demonstrated that this protocol offers at least comparable nucleic
acid quality, quantity and purity to those provided by commercial phenol-based or spin column systems and that are suitable to be used in PCR, RT-PCR and qPCR for virus and viroid detection. Because of its easy implementation and the use of safe and inexpensive reagents, it can be easily implemented to work in plant virus and viroid detection in different plant species.
© 2016 Elsevier B.V. All rights reserved |
Palabras claves : |
DNA; PCR; PLANT VIROID; PLANT VIRUS; PURIFICATION; RNA; RT-PCR. |
Thesagro : |
CITRUS. |
Asunto categoría : |
-- |
Marc : |
LEADER 02137naa a2200301 a 4500 001 1055729 005 2019-10-09 008 2016 bl uuuu u00u1 u #d 024 7 $a10.1016/j.jviromet.2016.08.011$2DOI 100 1 $aARRUABARRENA, A. 245 $aApplication of a simple and affordable protocol for isolating plant total nucleic acids for RNA and DNA virus detection.$h[electronic resource] 260 $c2016 500 $aArticle history: Received 30 May 2016 / Received in revised form 26 July 2016 / Accepted 14 August 2016 / Available online 16 August 2016. 520 $aABSTRACT. Standard molecular methods for plant virus diagnosis require the purification of RNA or DNA extracts from a large number of samples, with sufficient concentration and quality for their use in PCR, RT-PCR, or qPCR analysis. Most methods are laborious and use either hazardous and/or costly chemicals.Apreviously published protocol for RNA isolation from several plant species yields high amounts of good quality RNADNA mixture in a simple, safe and inexpensive manner. In the present work, this method was tested to obtain RNA-DNA extracts from leaves of tomato, potato and three species of citrus, and was compared with two commercial kits. The results demonstrated that this protocol offers at least comparable nucleic acid quality, quantity and purity to those provided by commercial phenol-based or spin column systems and that are suitable to be used in PCR, RT-PCR and qPCR for virus and viroid detection. Because of its easy implementation and the use of safe and inexpensive reagents, it can be easily implemented to work in plant virus and viroid detection in different plant species. © 2016 Elsevier B.V. All rights reserved 650 $aCITRUS 653 $aDNA 653 $aPCR 653 $aPLANT VIROID 653 $aPLANT VIRUS 653 $aPURIFICATION 653 $aRNA 653 $aRT-PCR 700 1 $aBENITEZ-GALEANO, M.J. 700 1 $aGIAMBIASI, M. 700 1 $aBERTALMIO, A. 700 1 $aCOLINA, R. 700 1 $aHERNÁNDEZ-RODRÍGUEZ, L. 773 $tJournal of Virological Methods, 2016$gv.237, p. 14-17.
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